Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Glutathione You Are The Healer Acetaminophen's Role in Autism and ADHD: A Mitochondrial Perspective As a naturopathic doctor and a mom, Tylenol is a hard no in my house and I want to explain why. , , Acetaminophen (Tylenol) is processed through the liver, and in that process it can lower g glutamyl cycle. Depletion of glutathione by acetaminophen leads to Download Scientific Diagram