Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Figure 1 from Acetaminophen toxicity and 5 oxoproline (pyroglutamic acid): a tale of two cycles, one an ATP depleting futile cycle and the other a useful cycle. Semantic Scholar Tylenol, Autism, And The Glutathione Connection OC Integrative Medicine How can I minimize liver damage while taking Tylenol (acetaminophen) in relation to glutathione levels? Tylenol Glutathione Kizer Wellness LLC